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xeno-free l7™ hpsc basal medium  (Lonza)


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    Lonza xeno-free l7™ hpsc basal medium
    <t>L7™</t> TFO2 hPSC <t>xeno-free</t> media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.
    Xeno Free L7™ Hpsc Basal Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l7+hpsc+basal+medium/pmc06981419-274-20-41?v=Lonza
    Average 90 stars, based on 1 article reviews
    xeno-free l7™ hpsc basal medium - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "End-to-End Platform for Human Pluripotent Stem Cell Manufacturing"

    Article Title: End-to-End Platform for Human Pluripotent Stem Cell Manufacturing

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21010089

    L7™ TFO2 hPSC xeno-free media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.
    Figure Legend Snippet: L7™ TFO2 hPSC xeno-free media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.

    Techniques Used: Cell Culture

    Schematic representation of the end-to-end microcarrier-based human pluripotent stem cells (hPSCs) expansion platform. This platform includes the following attributes: perfused, xeno-free, proprietary media, plastic microcarriers (MCs) coated with proprietary matrix, high fold expansion without cell passaging, and a closed concentration step. The two-dimensional (2D) seed train could be avoided by using cryopreserved cells as the inoculum.
    Figure Legend Snippet: Schematic representation of the end-to-end microcarrier-based human pluripotent stem cells (hPSCs) expansion platform. This platform includes the following attributes: perfused, xeno-free, proprietary media, plastic microcarriers (MCs) coated with proprietary matrix, high fold expansion without cell passaging, and a closed concentration step. The two-dimensional (2D) seed train could be avoided by using cryopreserved cells as the inoculum.

    Techniques Used: Passaging, Concentration Assay



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    <t>L7™</t> TFO2 hPSC <t>xeno-free</t> media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.
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    Image Search Results


    L7™ TFO2 hPSC xeno-free media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.

    Journal: International Journal of Molecular Sciences

    Article Title: End-to-End Platform for Human Pluripotent Stem Cell Manufacturing

    doi: 10.3390/ijms21010089

    Figure Lengend Snippet: L7™ TFO2 hPSC xeno-free media supports the microcarrier (MC)-based expansion of RTiPSC3B and RTiPSC4i human-induced pluripotent stem cells (hiPSCs) leading to >2 × 10 6 cells/mL ( A ) and >10 fold expansion ( B ) in 17 days. Small- and large-sized MCs support the cell growth ( C ) and expansion ( D ) of RTiPSC3B in L7™ TFO2 hPSC xeno-free media. Inoculating less cells/mL does not compromise cell yield, but leads to rapid cell growth and higher cell expansion of RTiPSC4i ( E , F ) and RTiPSC3B ( G , H ), which were cultured on small- and large-sized MCs. Coating MCs with the L7™ hPSC Matrix is essential for the attachment and expansion of hiPSCs in L7™ TFO2 hPSC medium. RTiPSC3B inoculated at 0.2 × 10 6 cells/mL with small-sized microcarriers ( I ) and LiPSC18R inoculated at 0.04 × 10 6 cells/mL with large-sized microcarriers ( J ). RTiPSC4i inoculated at 0.04 × 10 6 cells/mL with large-sized coated and uncoated microcarriers. Cell growth ( K ) and fold expansion ( L ) are shown.

    Article Snippet: It is comprised of recombinant, xeno-free and defined L7™ hPSC Matrix (Lonza, FP-5020, Walkersville, MD, USA) to enable cell attachment, xeno-free L7™ hPSC basal medium, xeno-free L7™ hPSC medium supplement and non-enzymatic passaging solutions: L7™ hPSC passaging solution (yields cell clumps, Lonza, FP-5013) or F3 hPSC passaging solution (yields single cells).

    Techniques: Cell Culture

    Schematic representation of the end-to-end microcarrier-based human pluripotent stem cells (hPSCs) expansion platform. This platform includes the following attributes: perfused, xeno-free, proprietary media, plastic microcarriers (MCs) coated with proprietary matrix, high fold expansion without cell passaging, and a closed concentration step. The two-dimensional (2D) seed train could be avoided by using cryopreserved cells as the inoculum.

    Journal: International Journal of Molecular Sciences

    Article Title: End-to-End Platform for Human Pluripotent Stem Cell Manufacturing

    doi: 10.3390/ijms21010089

    Figure Lengend Snippet: Schematic representation of the end-to-end microcarrier-based human pluripotent stem cells (hPSCs) expansion platform. This platform includes the following attributes: perfused, xeno-free, proprietary media, plastic microcarriers (MCs) coated with proprietary matrix, high fold expansion without cell passaging, and a closed concentration step. The two-dimensional (2D) seed train could be avoided by using cryopreserved cells as the inoculum.

    Article Snippet: It is comprised of recombinant, xeno-free and defined L7™ hPSC Matrix (Lonza, FP-5020, Walkersville, MD, USA) to enable cell attachment, xeno-free L7™ hPSC basal medium, xeno-free L7™ hPSC medium supplement and non-enzymatic passaging solutions: L7™ hPSC passaging solution (yields cell clumps, Lonza, FP-5013) or F3 hPSC passaging solution (yields single cells).

    Techniques: Passaging, Concentration Assay